Bethyl Laboratories

Rabbit anti-WDR91 Antibody Affinity Purified

Primary
Catalog #A301-563A
TargetWDR91
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IP
Detection of human WDR91 by western blot of immunoprecipitates.
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Product Description

WDR91 (WD repeat-containing 91) contains seven WD repeats and is a member of the WD family of proteins. The WD repeat is defined by four or more repeating units of a conserved core of approximately 40 amino acids ending with tryptophan-aspartic acid (WD). WD repeats may serve as sites of protein-protein interaction for adaptor proteins and facilitate multiprotein complex formation. WD proteins are involved in a variety of cellular processes. The function of WDR91 has not been characterized.

Specifications

Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 250 and 300
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetWDR91
Antigen Species
Human
Gene ID29062
Gene Symbol
WDR91
Gene Name
WD repeat domain 91
Uniprot IDA4D1P6
Protein Name
WD repeat-containing protein 91
Alternate Names
HSPC049  •  SORF1  •  SORF-1  •  WD repeat-containing protein 91
Product Information
Applications
IHC, IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA

Production & Epitope

Antibody was affinity purified using an epitope specific to WDR91 immobilized on solid support.

The epitope recognized by A301-563A maps to a region between residue 250 and 300 of human WD repeat domain 91 using the numbering given in entry NP_054868.2 (GeneID 29062).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.

Immunohistochemistry (IHC)1:500 - 1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate

Documents

Product DatasheetPDF · A301-563A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-563A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-563AOpen
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