Bethyl Laboratories
Rabbit anti-NUP96 Antibody Affinity Purified
Primary
Catalog #A301-784A
TargetNUP96
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
The nuclear pore complex (NPC) is a structure that spans the nuclear envelope and provides a conduit between the nucleus and cytoplasm for the nucleocytoplasmic transport of macromolecules. The main components of the NPC are nucleoporins. There are about 30 different human Nups that have been identified. Nup98 is a 98 kDa protein generated from a 186 kDa precursor. The proteolytic cleavage of the 186 kDa precursor also results in a 96 kDa nucleoporin, Nup96. Nup98 and Nup96 have been shown to interact with each other and both play a role in bidirectional transport across the NPC. The NUP98 gene localizes to tumor-suppressor region associated with several types of cancer.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 925 and 975
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetNUP96
Antigen Species
Human
Gene ID4928
Gene Symbol
NUP98
Gene Name
nucleoporin 98
Uniprot IDP52948
Protein Name
Nuclear pore complex protein Nup98-Nup96
Product Information
Applications
IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to NUP96 immobilized on solid support.
The epitope recognized by A301-784A maps to a region between residue 900 and 950 of human nucleoporin 96kDa using the numbering given in entry NP_057404.2 (GeneID 4928).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A301-784A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-784A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-784AOpen
Citations / Publications
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