Bethyl Laboratories
Rabbit anti-c-Rel Antibody Affinity Purified
Primary
Catalog #A301-825A
Targetc-Rel
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
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Product Description
c-Rel is one of five monomeric subunits that are part of the Rel family of proteins that dimerize to form the NF-kappa B (nuclear factor that binds the kappa immunoglobulin light chain gene enhancer) transcription factor. Members of the Rel family of proteins include NF-kappa B1/p50, NF-kappa B2/p52, RelA/p65, c-Rel, and RelB. Each Rel member bears a characteristic 300 amino acid N-terminal Rel homology domain (RHD). c-Rel was originally identified as the cellular version of the v-Rel oncogene derived from the avian reticuloendotheliosis retrovirus.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 325 and 375
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
Targetc-Rel
Antigen Species
Human
Gene ID5966
Gene Symbol
REL
Gene Name
REL proto-oncogene, NF-kB subunit
Uniprot IDQ04864
Protein Name
Proto-oncogene c-Rel
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to c-Rel immobilized on solid support.
The epitope recognized by A301-825A maps to a region between residue 325 and 375 of human reticuloendotheliosis viral oncogene homolog using the numbering given in entry NP_002899.1 (GeneID 5966).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A301-825A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-825A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-825AOpen
Citations / Publications
Services
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