Bethyl Laboratories
Rabbit anti-SET Antibody Affinity Purified
Primary
Catalog #A302-261A
TargetSET
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC, IP, WB
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Product Description
SET is a multi-functional protein involved in apoptosis, transcription, nucleosome assembly, and histone binding. SET was identified as part of a chromosomal rearrangement with NUP214/CAN in acute undifferentiated leukemia.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 150 and 200
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetSET
Antigen Species
Human
Gene ID6418
Gene Symbol
SET
Gene Name
SET nuclear proto-oncogene
Uniprot IDQ01105
Protein Name
Protein SET
Alternate Names
2PP2A • chromatin remodelling factor • HLA-DR-associated protein II • I2PP2A • I-2PP2A • IGAAD • inhibitor of granzyme A-activated DNase • inhibitor-2 of protein phosphatase-2A • IPP2A2 • MRD58 • PHAPII • phosphatase 2A inhibitor I2PP2A • protein phosphatase type 2A inhibitor • protein SET • SET nuclear oncogene • SET translocation (myeloid leukemia-associated) • TAF-I • TAF-IBETA • Template-activating factor I • Template-Activating Factor-I, chromatin remodelling factor
Product Information
Applications
IHC, IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to SET immobilized on solid support.
The epitope recognized by A302-261A maps to a region between residue 125 and 175 of human SET nuclear oncogene using the numbering given in entry NP_001116293.1 (GeneID 6418).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:500 - 1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A302-261A · Lot 3 (current lot)Open
Product DatasheetPDF · A302-261A · Lot 3 (current lot)Open
Safety Data SheetPDF · A302-261AOpen
Citations / Publications
Services
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