Bethyl Laboratories

Rabbit anti-SLM-2 Antibody Affinity Purified

Primary
Catalog #A303-191A
TargetSLM-2
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
Detection of human SLM-2 by western blot of immunoprecipitates.
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Product Description

Sam68-like mammalian protein 2 (SLM-2) is an RNA-binding protein that plays a role in the regulation of alternative splicing and influences mRNA splice site selection and exon inclusion. SLM-2 may play a role as a negative regulator of cell growth [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ O75525].

Specifications

Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1 and 50
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetSLM-2
Antigen Species
Human
Gene ID10656
Gene Symbol
KHDRBS3
Gene Name
KH RNA binding domain containing, signal transduction associated 3
Uniprot IDO75525
Protein Name
KH domain-containing, RNA-binding, signal transduction-associated protein 3
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA

Production & Epitope

Antibody was affinity purified using an epitope specific to SLM-2 immobilized on solid support.

The epitope recognized by A303-191A maps to a region between residue 1 and 50 of human Sam68-like Mammalian Protein 2 using the numbering given in entry NP_006549.1 (GeneID 10656).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.

Immunoprecipitation (IP)6 µg/mg lysate

Documents

Product DatasheetPDF · A303-191A · Lot 1 (current lot)Open
Product DatasheetPDF · A303-191A · Lot 1 (current lot)Open
Safety Data SheetPDF · A303-191AOpen

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