Bethyl Laboratories
Rabbit anti-PRDM10 Antibody Affinity Purified
Primary
Catalog #A303-204A
TargetPRDM10
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIP, WB
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Product Description
PR domain-containing protein 10 (PRDM10) is a transcription factor that contains C2H2-type zinc-fingers. It also contains a positive regulatory domain, which has been found in several other zinc-finger transcription factors including those involved in B cell differentiation and tumor suppression. Studies of the mouse counterpart suggest that this protein may be involved in the development of the central nerve system (CNS), as well as in the pathogenesis of neuronal storage disease.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1097 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetPRDM10
Antigen Species
Human
Gene ID56980
Gene Symbol
PRDM10
Gene Name
PR/SET domain 10
Uniprot IDQ9NQV6
Protein Name
PR domain zinc finger protein 10
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to PRDM10 immobilized on solid support.
The epitope recognized by A303-204A maps to a region between residue 1097 and 1147 of human PR Domain Containing 10 using the numbering given in entry Q9NQV6.2 (GeneID 56980).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A303-204A · Lot 1 (current lot)Open
Product DatasheetPDF · A303-204A · Lot 1 (current lot)Open
Safety Data SheetPDF · A303-204AOpen
Citations / Publications
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