Rabbit anti-FAM76B Antibody Affinity Purified

Specifications

Production & Epitope

Antibody was affinity purified using an epitope specific to FAM76B immobilized on solid support.

The epitope recognized by A303-368A maps to a region between residue 150 and 200 of human Family with Sequence Similarity 76, Member B using the numbering given in entry NP_653265.3 (GeneID 143684).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.