Bethyl Laboratories
Rabbit anti-INO80 Antibody Affinity Purified
Primary
Catalog #A303-370A
TargetINO80
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
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Product Description
DNA helicase INO80 (INO80) is a DNA helicase and probable main scaffold component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9ULG1].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1400 and 1450
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetINO80
Antigen Species
Human
Gene ID54617
Gene Symbol
INO80
Gene Name
INO80 complex subunit
Uniprot IDQ9ULG1
Protein Name
DNA helicase INO80
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to INO80 immobilized on solid support.
The epitope recognized by A303-370A maps to a region between residue 1400 and 1450 of human INO80 homolog using the numbering given in entry NP_060023.1 (GeneID 54617).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A303-370A · Lot 2 (current lot)Open
Product DatasheetPDF · A303-370A · Lot 2 (current lot)Open
Safety Data SheetPDF · A303-370AOpen
Citations / Publications
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