Bethyl Laboratories
Rabbit anti-STAT2 Antibody Affinity Purified
Primary
Catalog #A303-512A
TargetSTAT2
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IP, WB
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Product Description
Signal transducer and activator of transcription 2 (STAT2) is a signal transducer and activator of transcription that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P52630].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 801 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetSTAT2
Antigen Species
Human
Gene ID6773
Gene Symbol
STAT2
Gene Name
signal transducer and activator of transcription 2
Uniprot IDP52630
Protein Name
Signal transducer and activator of transcription 2
Product Information
Applications
IHC, IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to STAT2 immobilized on solid support.
The epitope recognized by A303-512A maps to a region between residue 801 and 851 of human Signal Transducer and Activator of Transcription 2 using the numbering given in entry NP_005410.1 (GeneID 6773).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A303-512A · Lot 1 (current lot)Open
Product DatasheetPDF · A303-512A · Lot 1 (current lot)Open
Safety Data SheetPDF · A303-512AOpen
Citations / Publications
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