Bethyl Laboratories
Rabbit anti-MMS21 Antibody Affinity Purified
Primary
Catalog #A304-126A
TargetMMS21
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
The human MMS21 (Methyl Methanesulfonate-Sensitivity protein 21) homolog is also known as E3 SUMO-protein ligase NSE2. MMS21 is an E3 SUMO-protein ligase component of the SMC5-SMC6 complex, a complex involved in DNA double-strand break repair by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q96MF7].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1 and 50
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetMMS21
Antigen Species
Human
Gene ID286053
Gene Symbol
NSMCE2
Gene Name
NSE2/MMS21 homolog, SMC5-SMC6 complex SUMO ligase
Uniprot IDQ96MF7
Protein Name
E3 SUMO-protein ligase NSE2
Alternate Names
C8orf36 • E3 SUMO-protein ligase NSE2 • E3 SUMO-protein transferase NSE2 • hMMS21 • methyl methanesulfonate sensitivity gene 21 • MMS21 • MMS21 homolog • Non-SMC element 2 homolog • non-SMC element 2, MMS21 homolog • non-structural maintenance of chromosomes element 2 homolog • NSE2 • NSMCE2/PVT1 fusion • PVT1/NSMCE2 fusion • zinc finger, MIZ-type containing 7 • ZMIZ7
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to MMS21 immobilized on solid support.
The epitope recognized by A304-126A maps to a region between residue 1 to 50 of human Non-SMC Element 2 using the numbering given in entry NP_775956.1 (GeneID 286053).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A304-126A · Lot 2 (current lot)Open
Product DatasheetPDF · A304-126A · Lot 2 (current lot)Open
Safety Data SheetPDF · A304-126AOpen
Citations / Publications
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