Bethyl Laboratories
Rabbit anti-CD43 Antibody Affinity Purified
Primary
Catalog #A304-393A
TargetCD43
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IP, WB
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Product Description
CD43 is a major sialoglycoprotein found on the surface of thymocytes, T lymphocytes, monocytes, granulocytes, and some B lymphocytes. It may be part of a physiologic ligand-receptor complex involved in T-cell activation. During T-cell activation, CD43 is actively removed from the T-cell-APC (antigen-presenting cell) contact site, suggesting a negative regulatory role in adaptive immune respons [taken from NCBI Entrez Gene (Gene ID: 6693)].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 350 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetCD43
Antigen Species
Human
Gene ID6693
Gene Symbol
SPN
Gene Name
sialophorin
Uniprot IDP16150
Protein Name
Leukosialin
Alternate Names
Product Information
Applications
IHC, IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to CD43 immobilized on solid support.
The epitope recognized by A304-393A maps to a region between residue 350 to 400 of human CD43 Molecule using the numbering given in entry NP_001025459.1 (GeneID 6693).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:500 - 1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A304-393A · Lot 1 (current lot)Open
Product DatasheetPDF · A304-393A · Lot 1 (current lot)Open
Safety Data SheetPDF · A304-393AOpen
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