Bethyl Laboratories
Rabbit anti-CD7 Antibody Affinity Purified
Primary
Catalog #A304-396A
TargetCD7
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IP, WB
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Product Description
CD7 is a transmembrane protein which is a member of the immunoglobulin superfamily. This protein is found on thymocytes and mature T cells. It plays an essential role in T-cell interactions and also in T-cell/B-cell interaction during early lymphoid development [taken from NCBI Entrez Gene (Gene ID: 924)].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 190 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetCD7
Antigen Species
Human
Gene ID924
Gene Symbol
CD7
Gene Name
CD7 molecule
Uniprot IDP09564
Protein Name
T-cell antigen CD7
Alternate Names
Product Information
Applications
IHC, IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to CD7 immobilized on solid support.
The epitope recognized by A304-396A maps to a region between residue 190 to 240 of human CD7 Molecule using the numbering given in entry NP_006128.1 (GeneID 924).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:500 - 1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:1000 - 1:5000
Documents
Product DatasheetPDF · A304-396A · Lot 1 (current lot)Open
Product DatasheetPDF · A304-396A · Lot 1 (current lot)Open
Safety Data SheetPDF · A304-396AOpen
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