Bethyl Laboratories
Rabbit anti-Aladin Antibody Affinity Purified
Primary
Catalog #A304-514A
TargetAladin
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC, IP, WB
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Product Description
Aladin is a member of the WD-repeat family of regulatory proteins and may be involved in normal development of the peripheral and central nervous system. Aladin is part of the nuclear pore complex and is anchored there by NDC1. Defects in the coding gene are a cause of achalasia-addisonianism-alacrima syndrome (AAAS), also called triple-A syndrome or Allgrove syndrome [taken from NCBI Entrez Gene (Gene ID: 8086)].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 496 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetAladin
Antigen Species
Human
Gene ID8086
Gene Symbol
AAAS
Gene Name
aladin WD repeat nucleoporin
Uniprot IDQ9NRG9
Protein Name
Aladin
Alternate Names
Product Information
Applications
IHC, IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to Aladin immobilized on solid support.
The epitope recognized by A304-514A maps to a region between residue 496 to 546 of human adrenocortical Insufficiency using the numbering given in entry NP_056480.1 (GeneID 8086).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:500 - 1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A304-514A · Lot 1 (current lot)Open
Product DatasheetPDF · A304-514A · Lot 1 (current lot)Open
Safety Data SheetPDF · A304-514AOpen
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