Bethyl Laboratories
Rabbit anti-COPB2 Antibody Affinity Purified
Primary
Catalog #A304-523A
TargetCOPB2
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC, IP, WB
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Product Description
In eukaryotic cells, protein transport between the endoplasmic reticulum and Golgi compartments is mediated in part by non-clathrin-coated vesicular coat proteins (COPs). Seven coat proteins have been identified, and they represent subunits of a complex known as coatomer. The subunits are designated alpha-COP, beta-COP, beta-prime-COP, gamma-COP, delta-COP, epsilon-COP, and zeta-COP [taken from NCBI Entrez Gene (Gene ID: 9276)].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 856 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetCOPB2
Antigen Species
Human
Gene ID9276
Gene Symbol
COPB2
Gene Name
coatomer protein complex subunit beta 2
Uniprot IDP35606
Protein Name
Coatomer subunit beta'
Product Information
Applications
IHC, IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to COPB2 immobilized on solid support.
The epitope recognized by A304-523A maps to a region between residue 856 to 906 of human Coatomer Protein Complex, Subunit Beta 2 using the numbering given in entry NP_004757.1 (GeneID 9276).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:1000 to 1:5000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:10,000 - 1:25,000
Documents
Product DatasheetPDF · A304-523A · Lot 2 (current lot)Open
Product DatasheetPDF · A304-523A · Lot 2 (current lot)Open
Safety Data SheetPDF · A304-523AOpen
Citations / Publications
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