Bethyl Laboratories
Rabbit anti-STRAP Antibody Affinity Purified
Primary
Catalog #A304-735A
TargetSTRAP
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIP, WB
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Product Description
The SMN complex plays a catalyst role in the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Serine-threonine kinase receptor-associated protein (STRAP) plays a role in the cellular distribution of the SMN complex. It negatively regulates TGF-beta signaling but positively regulates the PDPK1 kinase activity by enhancing its autophosphorylation and by significantly reducing the association of PDPK1 with 14-3-3 protein [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9Y3F4].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 300 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetSTRAP
Antigen Species
Human
Gene ID11171
Gene Symbol
STRAP
Gene Name
serine/threonine kinase receptor associated protein
Uniprot IDQ9Y3F4
Protein Name
Serine-threonine kinase receptor-associated protein
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to STRAP immobilized on solid support.
The epitope recognized by A304-735A maps to a region between residue 300 to 350 of human Serine/Threonine Kinase Receptor Associated Protein using the numbering given in entry NP_009109.3 (GeneID 11171).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A304-735A · Lot 1 (current lot)Open
Product DatasheetPDF · A304-735A · Lot 1 (current lot)Open
Safety Data SheetPDF · A304-735AOpen
Citations / Publications
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