Bethyl Laboratories
Rabbit anti-CD247/CD3Z Antibody Affinity Purified
Primary
Catalog #A305-170A
TargetCD247/CD3Z
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
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Product Description
T-cell surface glycoprotein CD3 zeta chain is encoded by the gene CD247, also known as CD3Z or CD3 zeta. CD247/CD3 zeta, together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and -epsilon, forms the T-cell receptor-CD3 complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response [taken from NCBI Entrez Gene (Gene ID: 919)].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 75 and 125
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetCD247/CD3Z
Antigen Species
Human
Gene ID919
Gene Symbol
CD247
Gene Name
CD247 molecule
Uniprot IDP20963
Protein Name
T-cell surface glycoprotein CD3 zeta chain
Alternate Names
CD antigen CD247 • CD247 antigen, zeta subunit • CD3H • CD3Q • CD3Z • CD3Z antigen, zeta polypeptide (TiT3 complex) • CD3-ZETA • CD3zeta chain • IMD25 • T3Z • T-cell antigen receptor complex, zeta subunit of CD3 • T-cell receptor T3 zeta chain • T-cell surface glycoprotein CD3 zeta chain • TCR zeta chain • TCRZ
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to CD247/CD3Z immobilized on solid support.
The epitope recognized by A305-170A maps to a region between residue 75 to 125 of human T-cell surface glycoprotein CD3 zeta chain using the numbering given in entry P20963.2 (GeneID 919).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A305-170A · Lot 1 (current lot)Open
Product DatasheetPDF · A305-170A · Lot 1 (current lot)Open
Safety Data SheetPDF · A305-170AOpen
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