Bethyl Laboratories
Mouse anti-Cytokeratin 19 Monoclonal Antibody [BA17]
Monoclonal Antibodies
Catalog #A500-036A
TargetCytokeratin 19
HostMouse
CloneBA17
ReactivityHuman
ApplicationICC, IHC, WB
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Product Description
Cytokeratin 19 is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells. Cytokeratin 19 is Involved in the organization of myofibers. Together with Cytokeratin 8, it helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P08727].
Specifications
Clonality
Monoclonal
Clone
BA17
Host
Mouse
Immunogen
Mammary organoids
Isotype
IgG1
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Purified
Concentration
100 µg/ml
Target Identity
TargetCytokeratin 19
Antigen Species
Human
Gene ID3880
Gene Symbol
KRT19
Gene Name
keratin 19
Uniprot IDP08727
Protein Name
Keratin, type I cytoskeletal 19
Alternate Names
Product Information
Applications
ICC, IHC, WB
Buffer
Phosphate Buffered Saline (PBS) with 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Monoclonal antibody was purified from cell culture supernatant.
Immunogen was human mammary organoids.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots are performed using Goat anti-Mouse IgG Heavy and Light Chain Antibody (Cat. No. A90-216P).
Immunocytochemistry (ICC)1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.
Immunohistochemistry (IHC)1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Western Blot (WB)1:1000
Documents
Product DatasheetPDF · A500-036A · Lot 1 (current lot)Open
Product DatasheetPDF · A500-036A · Lot 1 (current lot)Open
Product DatasheetPDF · A500-036A · Lot 1 (current lot)Open
Safety Data SheetPDF · A500-036AOpen
Services
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Full custom monoclonal development, from immunization through validation.
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Single-domain antibodies for structurally difficult targets a conventional monoclonal can't reach.
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