Bethyl Laboratories
Rabbit anti-HIF2-alpha Recombinant Monoclonal Antibody [BL-95-1A2]
Monoclonal Antibodies
Catalog #A700-003
TargetHIF2-alpha
HostRabbit
CloneBL-95-1A2
ReactivityHuman
ApplicationChIP, ICC, ICC-IF, IHC, IP, WB
Image 1 of 3
local_shipping
Order within 20 hours and 46 minutes for shipment by today
Product Description
The gene EPAS1 encodes for the transcription factor endothelial PAS domain-containing protein 1, and is induced as oxygen levels fall. Commonly called hypoxia inducible factor 2A (HIF2a), this protein is involved in the induction of oxygen regulated genes, binding to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. An important cancer biomarker, HIF2a may also play a role in Tibetan high-altitude adaptation (Am J Hum Genet. 2015 Jul 2; 97(1): 54–66.) and mutations in the EPAS1 gene are associated with erythrocytosis familial type 4 [taken from NCBI Entrez Gene (Gene ID: 2034) and UniProtKB (Entry: Q99814)].
Specifications
Clonality
Recombinant Monoclonal
Clone
BL-95-1A2
Host
Rabbit
Immunogen
Between 400 and 450
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Purified
Concentration
400 µg/ml
Target Identity
TargetHIF2-alpha
Antigen Species
Human
Gene ID2034
Gene Symbol
EPAS1
Gene Name
endothelial PAS domain protein 1
Uniprot IDQ99814
Protein Name
Endothelial PAS domain-containing protein 1
Alternate Names
basic-helix-loop-helix-PAS protein MOP2 • bHLHe73 • class E basic helix-loop-helix protein 73 • ECYT4 • endothelial PAS domain-containing protein 1 • EPAS-1 • HIF-1-alpha-like factor • HIF-1alpha-like factor • HIF2A • HIF-2-alpha • HIF2-alpha • HLF • hypoxia-inducible factor 2 alpha • Hypoxia-inducible factor 2-alpha • member of PAS protein 2 • MOP2 • PAS domain-containing protein 2 • PASD2
Product Information
Applications
ChIP, ICC, ICC-IF, IHC, IP, WB
Buffer
Phosphate Buffered Saline (PBS) with 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Recombinant antibody was purified from cell culture supernatant.
The epitope recognized by A700-003 maps to a region between residues 400 and 450 of human endothelial PAS domain protein 1 using the numbering given in entry NP_001421.2 (GeneID 2034).
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
ChIP-SeqA previous lot has qualified for ChIP-Seq. 10 - 40 µl per 30 µg chromatin.
Immunocytochemistry (ICC)1:200 - 1:1000. Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.
Immunocytochemistry-Immunofluoresence (ICC-IF)1:100 - 1:500. Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.
Immunohistochemistry (IHC)1:200 - 1:1000. Epitope retrieval with citrate buffer pH 6.0 for 20 minutes using a pressure cooker is recommended for FFPE tissue sections. Overnight incubations are suggested.
Immunoprecipitation (IP)20 µl/mg lysate
Western Blot (WB)1:1000
Documents
Product DatasheetPDF · A700-003 · Lot 4 (current lot)Open
Product DatasheetPDF · A700-003 · Lot 4 (current lot)Open
Product DatasheetPDF · A700-003 · Lot 160513Open
Safety Data SheetPDF · A700-003Open
Citations / Publications
Services
Antibody Conjugation
Antibody and conjugate formats developed together, built to your specification.
Request CustomizationCustom Monoclonal Antibody Discovery Services
Full custom monoclonal development, from immunization through validation.
Explore Monoclonal DevelopmentVHH Discovery
Single-domain antibodies for structurally difficult targets a conventional monoclonal can't reach.
Explore VHH DiscoveryResources
1