Bethyl Laboratories
Rabbit anti-CD4 Recombinant Monoclonal Antibody [BL-155-1C11]
Monoclonal Antibodies
Catalog #A700-015
TargetCD4
HostRabbit
CloneBL-155-1C11
ReactivityHuman
ApplicationFlow Cyt, IHC, IP, mIF, WB
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Product Description
CD4 is a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. CD4 is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system [taken from NCBI Entrez Gene (Gene ID: 920)].
Specifications
Clonality
Recombinant Monoclonal
Clone
BL-155-1C11
Host
Rabbit
Immunogen
Residues 26-397 (ECD)
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Purified
Concentration
1000 µg/ml
Target Identity
TargetCD4
Antigen Species
Human
Gene ID920
Gene Symbol
CD4
Gene Name
CD4 molecule
Uniprot IDP01730
Protein Name
T-cell surface glycoprotein CD4
Alternate Names
Product Information
Applications
Flow Cyt, IHC, IP, mIF, WB
Buffer
Phosphate Buffered Saline (PBS) with 0.09% Sodium Azide, BSA-Free
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Recombinant antibody was purified from cell culture supernatant.
Immunogen was a recombinant protein representing the extracellular domain of human CD4 (residues 26-397, NP_000607.1).
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Flow Cytometry (Flow Cyt)Live or cells fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 µl per 1 x 10^6 cells.
Immunohistochemistry (IHC)1:100 - 1:500. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µl/mg lysate
Multiplex Immunofluorescence (mIF)1:250
Western Blot (WB)1:1000
Documents
Product DatasheetPDF · A700-015 · Lot 8 (current lot)Open
Product DatasheetPDF · A700-015 · Lot 8 (current lot)Open
Safety Data SheetPDF · A700-015Open
Citations / Publications
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Resources
Standard Western Blot Protocol
Read the Protocol
Immunoprecipitation Protocol
Read the Protocol
Simple Western™ Resources
Explore Simple Western™
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