Bethyl Laboratories
Rabbit anti-CD3E Recombinant Monoclonal Antibody [BL-298-5D12]
Monoclonal Antibodies
Catalog #A700-016
TargetCD3E
HostRabbit
CloneBL-298-5D12
ReactivityHuman
ApplicationFlow Cyt, IHC, IHC-IF, IP, mIF, WB
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Product Description
CD3E is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The epsilon polypeptide plays an essential role in T-cell development [taken from NCBI Entrez Gene (Gene ID: 916)].
Specifications
Clonality
Recombinant Monoclonal
Clone
BL-298-5D12
Host
Rabbit
Immunogen
Between 157 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Purified
Concentration
50 µg/ml
Target Identity
TargetCD3E
Antigen Species
Human
Gene ID916
Gene Symbol
CD3E
Gene Name
CD3e molecule
Uniprot IDP07766
Protein Name
T-cell surface glycoprotein CD3 epsilon chain
Alternate Names
Product Information
Applications
Flow Cyt, IHC, IHC-IF, IP, mIF, WB
Buffer
Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Recombinant antibody was purified from cell culture supernatant.
Immunogen was a synthetic peptide representing a region of CD3E and maps to a region between residues 157 to 207 of human CD3e Molecule, Epsilon (CD3-TCR Complex) according to the numbering given in entry NP_000724.1 (GeneID 916).
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Flow Cytometry (Flow Cyt)Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 µl per 1 x 10^6 cells.
Immunocytochemistry (ICC)1:100 - 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
Immunohistochemistry (IHC)1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunohistochemistry-Immunofluoresence (IHC-IF)1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
Immunoprecipitation (IP)20 µl/mg lysate
Multiplex Immunofluorescence (mIF)1:250
Western Blot (WB)1:1000
Documents
Product DatasheetPDF · A700-016 · Lot 3 (current lot)Open
Product DatasheetPDF · A700-016 · Lot 3 (current lot)Open
Product DatasheetPDF · A700-016 · Lot 200218Open
Safety Data SheetPDF · A700-016Open
Citations / Publications
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Resources
Standard Western Blot Protocol
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Immunoprecipitation Protocol
Read the Protocol
Simple Western™ Resources
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