Bethyl Laboratories
Rabbit anti-CD247/CD3Z Recombinant Monoclonal Antibody [BL-336-1B2]
Monoclonal Antibodies
Catalog #A700-017
TargetCD247/CD3Z
HostRabbit
CloneBL-336-1B2
ReactivityHuman
ApplicationFlow Cyt, IHC, IHC-IF, IP, WB
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Product Description
T-cell surface glycoprotein CD3 zeta chain is encoded by the gene CD247, also known as CD3Z or CD3 zeta. CD247/CD3 zeta, together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and -epsilon, forms the T-cell receptor-CD3 complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response [taken from NCBI Entrez Gene (Gene ID: 919)].
Specifications
Clonality
Recombinant Monoclonal
Clone
BL-336-1B2
Host
Rabbit
Immunogen
Between 114 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Purified
Concentration
100 µg/ml
Target Identity
TargetCD247/CD3Z
Antigen Species
Human
Gene ID919
Gene Symbol
CD247
Gene Name
CD247 molecule
Uniprot IDP20963
Protein Name
T-cell surface glycoprotein CD3 zeta chain
Alternate Names
CD antigen CD247 • CD247 antigen, zeta subunit • CD3H • CD3Q • CD3Z • CD3Z antigen, zeta polypeptide (TiT3 complex) • CD3-ZETA • CD3zeta chain • IMD25 • T3Z • T-cell antigen receptor complex, zeta subunit of CD3 • T-cell receptor T3 zeta chain • T-cell surface glycoprotein CD3 zeta chain • TCR zeta chain • TCRZ
Product Information
Applications
Flow Cyt, IHC, IHC-IF, IP, WB
Buffer
Phosphate Buffered Saline (PBS) with 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Recombinant antibody was purified from cell culture supernatant.
Immunogen was a synthetic peptide representing a region between residue 150 and the C-terminus (residue 164) of human T-cell surface glycoprotein CD3 zeta chain using the numbering given in entry NP_932170.1 (GeneID 919).
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Western Blot (WB)1:1000
Immunoprecipitation (IP)20 µl/mg lysate
Immunohistochemistry (IHC)1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunohistochemistry-Immunofluoresence (IHC-IF)1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Flow Cytometry (Flow Cyt)Fixed in 4% formaldehyde and permeabilized with 90% methanol. 0.5 µl per 1 x 10^6 cells.
SW-Size1:10 - 1:250
Documents
Product DatasheetPDF · A700-017 · Lot 4 (current lot)Open
Product DatasheetPDF · A700-017 · Lot 4 (current lot)Open
Product DatasheetPDF · A700-017 · Lot 4 (current lot)Open
Safety Data SheetPDF · A700-017Open
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