Bethyl Laboratories
Rabbit anti-Granzyme B Recombinant Monoclonal Antibody [BLR022E]
Monoclonal Antibodies
Catalog #A700-022
TargetGranzyme B
HostRabbit
CloneBLR022E
ReactivityHuman
ApplicationICC, IHC, IHC-IF, mIF, WB
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Product Description
Granzyme B is a member of the granzyme subfamily of proteins, part of the peptidase S1 family of serine proteases. The preproprotein is secreted by natural killer (NK) cells and cytotoxic T lymphocytes (CTLs) and proteolytically processed to generate the active protease, which induces target cell apoptosis. This protein also processes cytokines and degrades extracellular matrix proteins, and these roles are implicated in chronic inflammation and wound healing [taken from NCBI Entrez Gene (Gene ID: 3002)]
Specifications
Clonality
Recombinant Monoclonal
Clone
BLR022E
Host
Rabbit
Immunogen
Residues 19-247 (mature protein)
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Purified
Concentration
50 µg/ml
Target Identity
TargetGranzyme B
Antigen Species
Human
Gene ID3002
Gene Symbol
GZMB
Gene Name
granzyme B
Uniprot IDP10144
Protein Name
Granzyme B
Alternate Names
C11 • cathepsin G-like 1 • CCPI • CGL1 • CGL-1 • CSPB • CSP-B • CTLA1 • CTLA-1 • CTSGL1 • cytotoxic serine protease B • cytotoxic T-lymphocyte proteinase 2 • fragmentin 2 • Fragmentin-2 • granzyme B • granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1) • Granzyme-2 • HLP • human lymphocyte protein • Lymphocyte protease • SECT • T-cell serine protease 1-3E
Product Information
Applications
ICC, IHC, IHC-IF, mIF, WB
Buffer
Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
The monoclonal antibody was purified from cell culture supernatant.
Immunogen was a recombinant protein representing human Granzyme B (residues 19-247, NP_004122.2).
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Flow Cytometry (Flow Cyt)Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 µl per 1 x 10^6 cells.
Immunocytochemistry (ICC)1:100 - 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
Immunohistochemistry (IHC)1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunohistochemistry-Immunofluoresence (IHC-IF)1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Multiplex Immunofluorescence (mIF)1:250
Western Blot (WB)1:1000
Documents
Product DatasheetPDF · A700-022 · Lot 3 (current lot)Open
Product DatasheetPDF · A700-022 · Lot 3 (current lot)Open
Product DatasheetPDF · A700-022 · Lot 171106Open
Safety Data SheetPDF · A700-022Open
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Resources
Standard Western Blot Protocol
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Immunoprecipitation Protocol
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