Bethyl Laboratories
Rabbit anti-LAG3 Recombinant Monoclonal Antibody [BLR027F]
Monoclonal Antibodies
Catalog #A700-027
TargetLAG3
HostRabbit
CloneBLR027F
ReactivityHuman
ApplicationFlow Cyt, ICC, IHC, IHC-IF, IP, mIF, WB
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Product Description
Lymphocyte-activation protein 3 belongs to Ig superfamily and contains 4 extracellular Ig-like domains. The LAG3 gene contains 8 exons. The sequence data, exon/intron organization, and chromosomal localization all indicate a close relationship of LAG3 to CD4 [taken from NCBI Entrez Gene (Gene ID: 3902)]
Specifications
Clonality
Recombinant Monoclonal
Clone
BLR027F
Host
Rabbit
Immunogen
Between 475 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Purified
Concentration
500 µg/ml
Target Identity
TargetLAG3
Antigen Species
Human
Gene ID3902
Gene Symbol
LAG3
Gene Name
lymphocyte activating 3
Uniprot IDP18627
Protein Name
Lymphocyte activation gene 3 protein
Alternate Names
Product Information
Applications
Flow Cyt, ICC, IHC, IHC-IF, IP, mIF, WB
Buffer
Phosphate Buffered Saline (PBS) with 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Recombinant antibody was purified from cell culture supernatant.
Immunogen was a peptide representing a region between residue 500 to 525 of human lymphocyte activation gene 3 protein (LAG3) using the numbering given in entry NP_002277.4 (GeneID 3902).
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Flow Cytometry (Flow Cyt)Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 µl per 1 x 10^6 cells.
Immunocytochemistry (ICC)1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
Immunohistochemistry (IHC)1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Immunohistochemistry-Immunofluoresence (IHC-IF)1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)12 µl/mg lysate
Multiplex Immunofluorescence (mIF)1:250
Western Blot (WB)1:1000
Documents
Product DatasheetPDF · A700-027 · Lot 3 (current lot)Open
Product DatasheetPDF · A700-027 · Lot 3 (current lot)Open
Product DatasheetPDF · A700-027 · Lot 240912Open
Safety Data SheetPDF · A700-027Open
Citations / Publications
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Resources
Standard Western Blot Protocol
Read the Protocol
Immunoprecipitation Protocol
Read the Protocol
Simple Western™ Resources
Explore Simple Western™
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