Bethyl Laboratories
Rabbit anti-VE-Cadherin Recombinant Monoclonal Antibody [BLR239K]
Monoclonal Antibodies
Catalog #A700-239
TargetVE-Cadherin
HostRabbit
CloneBLR239K
ReactivityMouse
ApplicationICC, IHC, IP, WB
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Product Description
Cadherins are calcium-dependent cell adhesion proteins that preferentially interact with themselves in a homophilic manner in adjacent cells. VE-Cadherin, or Cadherin-5 plays an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton and acts in concert with KRIT1 and MPP5 to establish and maintain correct endothelial cell polarity and vascular lumen. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P33151].
Specifications
Clonality
Recombinant Monoclonal
Clone
BLR239K
Host
Rabbit
Immunogen
Residues 25-595 (ECD)
Isotype
IgG
Reactivity
Mouse
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Purified
Concentration
500 µg/ml
Target Identity
TargetVE-Cadherin
Antigen Species
Mouse
Gene ID12562
Gene Symbol
Cdh5
Gene Name
cadherin 5
Uniprot IDP55284
Protein Name
Cadherin-5
Alternate Names
7B4 • 7B4/cadherin-5 • AA408225 • cadherin-5 • CD antigen CD144 • Cd144 • vascular endothelial cadherin • Vec • VEcad • VE-Cad • VE-cadherin • VECD
Product Information
Applications
ICC, IHC, IP, WB
Buffer
Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Recombinant antibody was purified from cell culture supernatant.
Immunogen was a recombinant protein representing the extracellular domain of mouse Cadherin-5 (aka VE-Cadherin, residues 25-595, NP_033998.2).
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunocytochemistry (ICC)1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.
Immunohistochemistry (IHC)1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)12 µl/mg lysate
Western Blot (WB)1:1000
Documents
Product DatasheetPDF · A700-239 · Lot 1 (current lot)Open
Product DatasheetPDF · A700-239 · Lot 1 (current lot)Open
Product DatasheetPDF · A700-239 · Lot 1 (current lot)Open
Safety Data SheetPDF · A700-239Open
Services
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