Bethyl Laboratories
Rabbit anti-IL-12B Recombinant Monoclonal Antibody [BLR322M]
Primary
Catalog #A700-322
TargetIL-12B
HostRabbit
CloneBLR322M
ReactivityHuman
ApplicationIP, WB
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Product Description
IL-12B is a subunit of interleukin 12, a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. Interleukin 12 is a disulfide-linked heterodimer composed of the 40 kD IL-12B, and the 35 kD subunit IL-12A. IL-12 is expressed by activated macrophages that serve as an essential inducer of Th1 cells development, and has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. [provided by RefSeq, Jul 2008; taken from NCBI Entrez Gene (Gene ID: 3593)].
Specifications
Clonality
Recombinant Monoclonal
Clone
BLR322M
Host
Rabbit
Immunogen
residues 23-328
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Purified
Concentration
1000 µg/ml
Target Identity
TargetIL-12B
Antigen Species
Human
Gene ID3593
Gene Symbol
IL12B
Gene Name
interleukin 12B
Uniprot IDP29460
Protein Name
Interleukin-12 subunit beta
Alternate Names
CLMF • CLMF p40 • CLMF2 • cytotoxic lymphocyte maturation factor 40 kDa subunit • IL-12 subunit p40 • IL12, subunit p40 • IL-12B • IMD28 • IMD29 • interleukin 12, p40 • interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40) • interleukin-12 beta chain • interleukin-12 subunit beta • natural killer cell stimulatory factor, 40 kD subunit • NK cell stimulatory factor chain 2 • NKSF • NKSF2
Product Information
Applications
IP, WB
Buffer
Phosphate Buffered Saline (PBS) with 0.09% Sodium Azide, BSA-Free
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Recombinant antibody was purified from cell culture supernatant.
Immunogen was a recombinant protein representing human IL-12B (residues 23-328, NP_002178.2).
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Western Blot (WB)1:1,000
Immunoprecipitation (IP)6 µl/mg lysate
Documents
Product DatasheetPDF · A700-322 · Lot 1 (current lot)Open
Product DatasheetPDF · A700-322 · Lot 1 (current lot)Open
Safety Data SheetPDF · A700-322Open
Services
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