Bethyl Laboratories
Rabbit anti-AMPK alpha 1 IHC Antibody Affinity Purified
Primary
Catalog #IHC-00002
TargetAMPK alpha 1
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC
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Product Description
AMP-activated protein kinase (AMPK) is a heterotrimeric protein made up of a catalytic alpha subunit, and a regulatory beta and gamma subunit. There are two distinct genes for the alpha subunit, alpha1 and alpha2. AMPK alpha 1 is the serine/threonine kinase catalytic subunit of the AMPK. AMPK is responsible for regulating fatty acid and cholesterol synthesis. AMPK is activated in response to a wide variety of metabolic stresses and switches off anabolic pathways when ATP levels are depleted.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 375 and 425
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
50 µg/ml
Target Identity
TargetAMPK alpha 1
Antigen Species
Human
Gene ID5562
Gene Symbol
PRKAA1
Gene Name
protein kinase AMP-activated catalytic subunit alpha 1
Uniprot IDQ13131
Protein Name
5'-AMP-activated protein kinase catalytic subunit alpha-1
Alternate Names
5'-AMP-activated protein kinase catalytic subunit alpha-1 • 5'-AMP-activated protein kinase, catalytic alpha-1 chain • ACACA kinase • acetyl-CoA carboxylase kinase • AMP -activate kinase alpha 1 subunit • AMP-activated protein kinase, catalytic, alpha-1 • AMPK • AMPK alpha 1 • AMPK subunit alpha-1 • AMPKa1 • HMGCR kinase • hydroxymethylglutaryl-CoA reductase kinase • protein kinase, AMP-activated, alpha 1 catalytic subunit • tau-protein kinase PRKAA1
Product Information
Applications
IHC
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to AMPK alpha 1 immobilized on solid support.
The epitope recognized by IHC-00002 maps to a region between residue 350 and 400 of human 5'-AMP-activated Protein Kinase, catalytic Alpha-1 chain using the numbering given in entry BAA36547.1 (GeneID 5562).
Applications
Epitope exposure is not recommended.
Epitope exposure with citrate buffer will hinder staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.
Immunohistochemistry (IHC)1:100 - 1:500
Documents
Safety Data SheetPDF · IHC-00002Open
Product DatasheetPDF · IHC-00002 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00002 · Lot 1 (current lot)Open
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