Bethyl Laboratories
Rabbit anti-gamma-H2AX IHC Antibody Affinity Purified
Primary
Catalog #IHC-00059
Targetgamma-H2AX
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC
Image 1 of 2
local_shipping
Order within 3 hours and 29 minutes for shipment by today
Product Description
H2AX is a member of the histone H2A family. The four core histones involved in the formation of the nucleosome structure of compacted chromatin are H2A, H2B, H3, and H4. H2AX may function to facilitate DNA repair, and recent studies have shown that H2AX is required for the maintenance of genomic stability.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Surrounding serine 139
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
100 µg/ml
Target Identity
Targetgamma-H2AX
Antigen Species
Human
Gene ID3014
Gene Symbol
H2AFX
Gene Name
H2A histone family member X
Uniprot IDP16104
Protein Name
Histone H2AX
Alternate Names
H2A.X • H2A/X • H2AX • H2AX histone • histone H2A.x • histone H2AX
Product Information
Applications
IHC
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to gamma-H2AX immobilized on solid support.
The epitope recongized by IHC-00059 maps to a region surrounding phosphorylated serine 139 of human histone H2AX using the numbering given for Swiss-Prot entry P16104 (GeneID 3014).
Applications
Formalin-Fixed Paraffin-Embedded Tissue 1:100 - 1:500:
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Formaldehyde-Fixed Cells 1:100 - 1:500.
Immunocytochemistry (ICC)1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunohistochemistry (IHC)1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Documents
Safety Data SheetPDF · IHC-00059Open
Product DatasheetPDF · IHC-00059 · Lot 10 (current lot)Open
Product DatasheetPDF · IHC-00059 · Lot 10 (current lot)Open
Citations / Publications
Services
Antibody Conjugation
Antibody and conjugate formats developed together, built to your specification.
Request CustomizationCustom Monoclonal Antibody Discovery Services
Full custom monoclonal development, from immunization through validation.
Explore Monoclonal DevelopmentVHH Discovery
Single-domain antibodies for structurally difficult targets a conventional monoclonal can't reach.
Explore VHH DiscoveryResources
1