Bethyl Laboratories
Rabbit anti-CENP-B IHC Antibody Affinity Purified
Primary
Catalog #IHC-00064
TargetCENP-B
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationICC-IF
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Product Description
CENP-B is a centromeric protein that specifically binds the CENP-B box, a 17-base pair DNA sequence found in a subset of alpha-satellite DNA repeats. CENP-B associates with CENP-A and CENP-C in centromere specific nucleosomes to form the pre-kinetochore. CENP-B auto-Antibodies have been identified in sera from patients with autoimmune diseases.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 549 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
250 µg/ml
Target Identity
TargetCENP-B
Antigen Species
Human
Gene ID1059
Gene Symbol
CENPB
Gene Name
centromere protein B
Uniprot IDP07199
Protein Name
Major centromere autoantigen B
Alternate Names
Product Information
Applications
ICC-IF
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to CENP-B immobilized on solid support.
The epitope recognized by IHC-00064 maps to a region between residues 540 and the C-terminus (residue 599) of human Centromere Protein B using the numbering given in entry SwissProt entry P07199 (GeneID 1059).
Applications
Formaldehyde-Fixed Cells 1:100 - 1:500:
Permeabilization with Triton-X 100 is recommended.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.
Immunocytochemistry-Immunofluoresence (ICC-IF)1:100 - 1:500
Documents
Safety Data SheetPDF · IHC-00064Open
Product DatasheetPDF · IHC-00064 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00064 · Lot 1 (current lot)Open
Citations / Publications
Services
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