Bethyl Laboratories
Rabbit anti-Phospho MCM2 (S53) IHC Antibody Affinity Purified
Primary
Catalog #IHC-00069
TargetMCM2 Phospho (S53)
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC
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Product Description
The MCM (Mini-Chromosome Maintenance) complex is a key component of the pre-replication complex involved in replication licensing which restricts DNA replication to only once per cell cycle. The MCM complex is a heterohexamer of MCM2, MCM3, MCM4, MCM5, MCM6, and MCM7. MCM2 interacts with the core MCM complex (MCM4, 6, and 7) to regulate its helicase activity.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Surrounding serine 53
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
250 µg/ml
Target Identity
TargetMCM2 Phospho (S53)
Antigen Species
Human
Gene ID4171
Gene Symbol
MCM2
Gene Name
minichromosome maintenance complex component 2
Uniprot IDP49736
Protein Name
DNA replication licensing factor MCM2
Product Information
Applications
IHC
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to Phospho-MCM2 (S53) immobilized on solid support.
Immunogen for IHC-00069 was a synthetic phosphorylated peptide, which represented a portion of human Minichromosomal Maintenance Deficient 2 surrounding Serine 53 according to the numbering given in entry NP_004517.2 (GeneID 4171).
Applications
TBS buffer is recommended.
Epitope exposure is recommended.
Epitope exposure with citrate or tris-EDTA pH9.0 buffer will enhance staining.
Likely to work with frozen sections.
Immunohistochemistry (IHC)1:100 - 1:500
Documents
Safety Data SheetPDF · IHC-00069Open
Product DatasheetPDF · IHC-00069 · Lot 3 (current lot)Open
Product DatasheetPDF · IHC-00069 · Lot 3 (current lot)Open
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