Bethyl Laboratories
Rabbit anti-PLK1 IHC Antibody Affinity Purified
Primary
Catalog #IHC-00071
TargetPLK1
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationICC-IF
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Product Description
Polo-like kinase 1 (PLK1) activity is central to the progression through mitosis. PLK1 plays an essential role in spindle formation and chromosome segregation. Recent studies suggest that PLK1 is specifically involved in anaphase spindle elongation, Rho localization to the contractile ring and cytokinesis.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 325 and 375
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
250 µg/ml
Target Identity
TargetPLK1
Antigen Species
Human
Gene ID5347
Gene Symbol
PLK1
Gene Name
polo like kinase 1
Uniprot IDP53350
Protein Name
Serine/threonine-protein kinase PLK1
Product Information
Applications
ICC-IF
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to PLK1 immobilized on solid support.
The epitopes recognized by IHC-00071 maps to a region between residues 325 and 375 of human Polo-like kinase using the numbering given in SwissProt entry P53350 (GeneID 5347).
Applications
Formaldehyde-Fixed Cells 1:100 - 1:500:
Permeabilization with Triton-X 100 is recommended.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.
Immunocytochemistry-Immunofluoresence (ICC-IF)1:100 - 1:500
Documents
Safety Data SheetPDF · IHC-00071Open
Product DatasheetPDF · IHC-00071 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00071 · Lot 1 (current lot)Open
Citations / Publications
Services
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