Bethyl Laboratories
Rabbit anti-hSET1 Antibody Affinity Purified
Primary
Catalog #A300-290A
TargethSET1
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
hSET1 is a subunit of a human Set1-like histone methyltransferase (HMT) complex. The Set-1 like complexes are responsible for mono-, di-, and tri-methylation of histone H3 K4. The Set class of proteins takes its name from the 130-140 aa motif SET domain identified in drosophila proteins. hSET1 associates with a multitude of proteins in a Set1-like complex which includes hSet1, Ash2L, RBBP5,WDR5, hDPY-30, hSwd2, CXXC1, and HCF1.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1657 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargethSET1
Antigen Species
Human
Gene ID9739
Gene Symbol
SETD1A
Gene Name
SET domain containing 1A
Uniprot IDO15047
Protein Name
Histone-lysine N-methyltransferase SETD1A
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to hSET1 immobilized on solid support.
The epitope recognized by A300-290A maps to a region between residue 1675 and the C-terminus (residue 1709) of human hSET1 (KIAA0339) using the numbering given in TrEMBL entry O15047 (GeneID 9739).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A300-290A · Lot 2 (current lot)Open
Product DatasheetPDF · A300-290A · Lot 2 (current lot)Open
Safety Data SheetPDF · A300-290AOpen
Citations / Publications
Services
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