Bethyl Laboratories
Rabbit anti-FUS Antibody Affinity Purified
Primary
Catalog #A300-294A
TargetFUS
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC, IP, WB
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Product Description
FUS has been identified as a frequent translocation fusion partner of various transcription factors. FUS fusion genes have been shown to be associated with multiple tumor types which include liposarcoma, leukemia, histocytoma, and sarcoma. FUS is a multifunctional RNA-binding protein that associates with the nuclear matrix and Cajal bodies and appears to play a role in splicesome assembly, pre-mRNA splicing, DNA repair, transcriptional regulation and homologous recombination.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 476 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetFUS
Antigen Species
Human
Gene ID2521
Gene Symbol
FUS
Gene Name
FUS RNA binding protein
Uniprot IDP35637
Protein Name
RNA-binding protein FUS
Alternate Names
75 kDa DNA-pairing protein • ALS6 • ETM4 • FUS/ERG chimeric protein • FUS/ERG fusion protein • FUS1 • fused in sarcoma • fusion gene in myxoid liposarcoma • fus-like protein • heterogeneous nuclear ribonucleoprotein P2 • HNRNPP2 • oncogene FUS • oncogene TLS • POMP75 • RNA-binding protein FUS • TLS • translocated in liposarcoma protein
Product Information
Applications
IHC, IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to FUS immobilized on solid support.
The epitope recognized by A300-294A maps to a region between residue 500 and the C-terminus (residue 526) of human fusion (involved in t(12;16) in malignant liposarcoma) using the numbering given in SwissProt entry P35637 (GeneID 2521).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:1000 - 1:10,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:10,000 - 1:50,000
Documents
Product DatasheetPDF · A300-294A · Lot 2 (current lot)Open
Product DatasheetPDF · A300-294A · Lot 2 (current lot)Open
Safety Data SheetPDF · A300-294AOpen
Citations / Publications
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