Bethyl Laboratories
Rabbit anti-KIAA0082 Antibody Affinity Purified
Primary
Catalog #A300-303A
TargetKIAA0082
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
KIAA0082 was first identified as a hypothetical protein predicted from the in silico analysis of long cDNAs isolated in the Kazusa cDNA sequencing project. KIAA0082 contains a G-patch and WW domain and several predicted phosphoserine sites.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1 and 50
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetKIAA0082
Antigen Species
Human
Gene ID23070
Gene Symbol
CMTR1
Gene Name
cap methyltransferase 1
Uniprot IDQ8N1G2
Protein Name
Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 1
Alternate Names
Cap methyltransferase 1 • cap1 2'O-ribose methyltransferase 1 • cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 1 • FtsJ methyltransferase domain containing 2 • ftsJ methyltransferase domain-containing protein 2 • FTSJD2 • hMTr1 • interferon-stimulated gene 95 kDa protein • ISG95 • KIAA0082 • MTr1 • S-adenosyl-L-methionine-dependent methyltransferase FTSJD2
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to KIAA0082 immobilized on solid support.
The epitope recognized by A300-303A maps to a region between residues 1 and 50 of human KIAA0082 using the numbering given in TrEMBL entry Q8N1G2 (GeneID 23070).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A300-303A · Lot 2 (current lot)Open
Product DatasheetPDF · A300-303A · Lot 2 (current lot)Open
Safety Data SheetPDF · A300-303AOpen
Citations / Publications
Services
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