Bethyl Laboratories
Rabbit anti-KIAA0082 Antibody Affinity Purified
Primary
Catalog #A300-304A
TargetKIAA0082
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC, IP, WB
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Product Description
KIAA0082 was first identified as a hypothetical protein predicted from the in silico analysis of long cDNAs isolated in the Kazusa cDNA sequencing project. KIAA0082 contains a G-patch and WW domain and several predicted phosphoserine sites.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 125 and 175
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetKIAA0082
Antigen Species
Human
Gene ID23070
Gene Symbol
CMTR1
Gene Name
cap methyltransferase 1
Uniprot IDQ8N1G2
Protein Name
Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 1
Alternate Names
Cap methyltransferase 1 • cap1 2'O-ribose methyltransferase 1 • cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 1 • FtsJ methyltransferase domain containing 2 • ftsJ methyltransferase domain-containing protein 2 • FTSJD2 • hMTr1 • interferon-stimulated gene 95 kDa protein • ISG95 • KIAA0082 • MTr1 • S-adenosyl-L-methionine-dependent methyltransferase FTSJD2
Product Information
Applications
IHC, IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to KIAA0082 immobilized on solid support.
The epitope recognized by A300-304A maps to a region between residues 100 and 150 of human KIAA0082 using the numbering given in TrEMBL entry Q8N1G2 (GeneID 23070).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:500 - 1:2000. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:5000 - 1:25,000
Documents
Product DatasheetPDF · A300-304A · Lot 1 (current lot)Open
Product DatasheetPDF · A300-304A · Lot 1 (current lot)Open
Safety Data SheetPDF · A300-304AOpen
Citations / Publications
Services
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