Bethyl Laboratories
Rabbit anti-TAFII68 Antibody Affinity Purified
Primary
Catalog #A300-308A
TargetTAFII68
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IP, WB
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Product Description
The TFIID complex is important for coordinating the pre-initiation complex and transcription by RNA Pol II. The complex contains the TFIID protein, TATA binding protein (TBP) and multiple TBP-associated factors (TAFs). TAFII68 was identified as a subunit of the TFIID complex, but has also been found to be associated with the Pol II multiprotein complex. TAFII68 is part of the TET family of proteins that can bind RNA and single-stranded DNA. This family also includes the human sarcoma-associated proteins EWS and TLS. The association of this family of proteins with Pol II suggests that they may bridge the processes of transcription initiation and elongation. The N-terminus domain of TAFII68 in gene fusions has been shown to have oncogenic properties, similar to the chromosomal translocation associated with the EWS gene.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 200 and 250
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetTAFII68
Antigen Species
Human
Gene ID8148
Gene Symbol
TAF15
Gene Name
TATA-box binding protein associated factor 15
Uniprot IDQ92804
Protein Name
TATA-binding protein-associated factor 2N
Alternate Names
68 kDa TATA-binding protein-associated factor • Npl3 • RBP56 • RBP56/CSMF fusion • RNA-binding protein 56 • TAF(II)68 • TAF15 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 68kDa • TAF2N • TAFII68 • TATA box binding protein (TBP)-associated factor, RNA polymerase II, N, 68kD (RNA-binding protein 56) • TATA box-binding protein-associated factor 2N (RNA-binding protein 56) • TATA-binding protein-associated factor 2N • TBP-associated factor 15
Product Information
Applications
IHC, IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to TAFII68 immobilized on solid support.
The epitope recognized by A300-308A maps to a region between residues 200 and 250 of human TAFII68 using the numbering given in Swiss-Prot entry Q92804 (GeneID 8148).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:1000 - 1:5000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:5000 - 1:20,000
Documents
Product DatasheetPDF · A300-308A · Lot 1 (current lot)Open
Product DatasheetPDF · A300-308A · Lot 1 (current lot)Open
Safety Data SheetPDF · A300-308AOpen
Citations / Publications
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