Bethyl Laboratories
Rabbit anti-CoAA Antibody Affinity Purified
Primary
Catalog #A300-331A
TargetCoAA
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, WB
Image 1 of 2
local_shipping
Order within 13 hours and 27 minutes for shipment by Tuesday, November 3
Product Description
CoAA (coactivator activator) is also known as RBM14 (RNA binding motif protein 14) because of the presence of two RNA recognition motifs (RRMs) in its N-terminus. CoAA has been observed to mediate the coupled processes of transcription and splicing in a promoter-specific manner.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 125 and 175
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetCoAA
Antigen Species
Human
Gene ID10432
Gene Symbol
RBM14
Gene Name
RNA binding motif protein 14
Uniprot IDQ96PK6
Protein Name
RNA-binding protein 14
Product Information
Applications
IHC, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to CoAA immobilized on solid support.
The epitope recognized by A300-331A maps to a region between residues 150 and 200 of human Coactivator Activator (RNA Binding Motif Protein 14) using the numbering given in entry NP_006319.1 (GeneID 10432).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Immunohistochemistry (IHC)1:250 - 1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Western Blot (WB)1:1000 - 1:10,000
Documents
Product DatasheetPDF · A300-331A · Lot 1 (current lot)Open
Product DatasheetPDF · A300-331A · Lot 1 (current lot)Open
Safety Data SheetPDF · A300-331AOpen
Citations / Publications
Services
Antibody Conjugation
Antibody and conjugate formats developed together, built to your specification.
Request CustomizationCustom Monoclonal Antibody Discovery Services
Full custom monoclonal development, from immunization through validation.
Explore Monoclonal DevelopmentVHH Discovery
Single-domain antibodies for structurally difficult targets a conventional monoclonal can't reach.
Explore VHH DiscoveryResources
1