Bethyl Laboratories
Rabbit anti-JLP Antibody Affinity Purified
Primary
Catalog #A300-337A
TargetJLP
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC, IP, WB
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Product Description
JLP (JNK-interacting leucine zipper protein) has been identified as a scaffolding protein that functions to position mitogen-activated protein kinases (MAPKs) into specific signaling modules so that successive phosphorylation events of the MAPK pathway can occur. JLP is also known as sperm associated antigen 9 (SPAG9) due to its abundant expression in testicular haploid germ cells.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 775 and 825
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetJLP
Antigen Species
Human
Gene ID9043
Gene Symbol
SPAG9
Gene Name
sperm associated antigen 9
Uniprot IDO60271
Protein Name
C-Jun-amino-terminal kinase-interacting protein 4
Alternate Names
cancer/testis antigen 89 • c-Jun NH2-terminal kinase-associated leucine zipper protein • C-Jun-amino-terminal kinase-interacting protein 4 • CT89 • HLC4 • HLC6 • HLC-6 • human lung cancer oncogene 6 protein • JIP4 • JIP-4 • JLP • JNK interacting protein • JNK/SAPK-associated protein • JNK-associated leucine-zipper protein • JNK-interacting protein 4 • lung cancer oncogene 4 • Max-binding protein • mitogen-activated protein kinase 8-interacting protein 4 • PHET • PIG6 • proliferation-inducing gene 6 • Proliferation-inducing protein 6 • protein highly expressed in testis • sperm associated antigen 9 variant 5 • sperm surface protein • Sperm-associated antigen 9 • Sperm-specific protein • sunday driver 1
Product Information
Applications
IHC, IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to JLP immobilized on solid support.
The epitope recognized by A300-337A maps to a region between residue 775 and 825 of human JNK/SAPK-Associated Protien using the numbering given in entry NP_003962.3 (GeneID 9043).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:500 - 1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:1000 - 1:10,000
Documents
Product DatasheetPDF · A300-337A · Lot 2 (current lot)Open
Product DatasheetPDF · A300-337A · Lot 2 (current lot)Open
Safety Data SheetPDF · A300-337AOpen
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