Bethyl Laboratories
Rabbit anti-Roquin Antibody Affinity Purified
Primary
Catalog #A300-514A
TargetRoquin
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIP, WB
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Product Description
The conjugation of ubiquitin to proteins is an important means to regulate protein activity for many cellular processes by tagging them for degradation. Targeting of proteins by ubiquitination occurs in three main steps that involve the activation of ubiquitin by E1 enzymes, the carrying of ubiquitin to the target protein by E2 enzymes and the ligation of ubiquitin to the target protein by E3 enzymes. Roquin is a member of the RING type E3 ubiquitin ligases. Roquin possesses a CCCH zinc-finger found in RNA-binding proteins and is implicated to play a role in the development of autoimmune disease.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 500 and 550
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetRoquin
Antigen Species
Human
Gene ID149041
Gene Symbol
RC3H1
Gene Name
ring finger and CCCH-type domains 1
Uniprot IDQ5TC82
Protein Name
Roquin-1
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to Roquin immobilized on solid support.
The epitope recognized by A300-514A maps to a region between residues 500 and 550 of human Ring Finger and CCCH-type finder domains 1 using the numbering given in entry NP_742068.1 (GeneID 149041).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:500 - 1:5000
Documents
Product DatasheetPDF · A300-514A · Lot 2 (current lot)Open
Product DatasheetPDF · A300-514A · Lot 2 (current lot)Open
Safety Data SheetPDF · A300-514AOpen
Citations / Publications
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