Bethyl Laboratories
Rabbit anti-DDX19 Antibody Affinity Purified
Primary
Catalog #A300-547A
TargetDDX19
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC, IP, WB
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Product Description
DDX19 is a member of the DEAD box family of proteins that possesses several conserved motifs which include the highly conserved DEAD (Asp-Glu-Ala-Asp) amino acid sequence motif. The major activity of DEAD box proteins is to function as ATP-dependent RNA helicases. As helicases, DEAD proteins play an important role in all aspects of RNA metabolism and function which include pre-mRNA splicing, RNA synthesis, RNA degradation, RNA export, RNA translation, RNA secondary structure formation, ribosome biogenesis, and the assembly of RNP complexes. DDX19 may be involved in mRNA transport.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 429 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetDDX19
Antigen Species
Human
Gene ID11269
Gene Symbol
DDX19B
Gene Name
DEAD-box helicase 19B
Uniprot IDQ9UMR2
Protein Name
ATP-dependent RNA helicase DDX19B
Alternate Names
ATP-dependent RNA helicase DDX19 • ATP-dependent RNA helicase DDX19B • DBP5 • DDX19 • DEAD (Asp-Glu-Ala-As) box polypeptide 19B • DEAD (Asp-Glu-Ala-Asp) box polypeptide 19B • DEAD box protein 19B • DEAD box RNA helicase DEAD5 • DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 19 (Dbp5, yeast, homolog) • DEAD-box protein 5 • DEAD-box RNA helicase DEAD5 • RNAh • yeast Dbp5 homolog
Product Information
Applications
IHC, IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to DDX19 immobilized on solid support.
The epitope recognized by A300-547A maps to a region between residue 450 and the C-terminus (residue 479) of human DEAD (Asp-Glu-Ala-Asp) box polypeptide 19B using the numbering given in entry NP_009173.1 (GeneID 11269). The epitope is highly conserved with DDX19A (GeneID 55308), but crossreactivity of the antibody with this homolog has not been assessed.
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:100 to 1:500. Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:1000 - 1:10,000
Documents
Product DatasheetPDF · A300-547A · Lot 2 (current lot)Open
Product DatasheetPDF · A300-547A · Lot 2 (current lot)Open
Safety Data SheetPDF · A300-547AOpen
Citations / Publications
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