Bethyl Laboratories
Rabbit anti-NONO Antibody Affinity Purified
Primary
Catalog #A300-587A
TargetNONO
HostRabbit
ClonalityPolyclonal
ReactivityRat, Mouse, Human
ApplicationIHC, IP, WB
Image 1 of 5
local_shipping
Order within 12 hours and 3 minutes for shipment by today
Product Description
Non-POU-domain-containing octamer binding protein (NONO) is a member of the DBHS (drosophila behavior, human splicing) domain-containing family and is an RNA- and DNA- binding protein. NONO and other DBHS domain-containing proteins are multifunctional and are reported to be involved in transcriptional regulation, mRNA processing, and DNA non-homologous end joining (NHEJ). NONO functions as a coregulator of the androgen receptor (AR) and also regulates cAMP transcriptional activity by interacting with gene promoter elements. NONO is also involved in pre-mRNA splicing through an interaction with U5 snRNA and can stimulate DNA nonhomologous end joining (NHEJ) through the interaction with ku70/G22p and ku80/XRCC5 dimers.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 350 and 400
Isotype
IgG
Reactivity
Rat, Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetNONO
Antigen Species
Human
Gene ID4841
Gene Symbol
NONO
Gene Name
non-POU domain containing, octamer-binding
Uniprot IDQ15233
Protein Name
Non-POU domain-containing octamer-binding protein
Alternate Names
54 kDa nuclear RNA- and DNA-binding protein • 55 kDa nuclear protein • DNA-binding p52/p100 complex, 52 kDa subunit • MRXS34 • NMT55 • NonO protein • non-POU domain-containing octamer (ATGCAAAT) binding protein • non-POU domain-containing octamer-binding protein • NRB54 • P54 • p54(nrb) • P54NRB • PPP1R114 • protein phosphatase 1, regulatory subunit 114
Product Information
Applications
IHC, IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to NONO immobilized on solid support.
The epitope recognized by A300-587A maps to a region between residues 350 and 400 of human Non-POU Domain Containing, Octamer-binding using the numbering given in entry NP_031389.3 (GeneID 4841).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µl/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Immunohistochemistry (IHC)1:200 - 1:1000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Documents
Product DatasheetPDF · A300-587A · Lot 4 (current lot)Open
Product DatasheetPDF · A300-587A · Lot 4 (current lot)Open
Safety Data SheetPDF · A300-587AOpen
Citations / Publications
Services
Antibody Conjugation
Antibody and conjugate formats developed together, built to your specification.
Request CustomizationCustom Monoclonal Antibody Discovery Services
Full custom monoclonal development, from immunization through validation.
Explore Monoclonal DevelopmentVHH Discovery
Single-domain antibodies for structurally difficult targets a conventional monoclonal can't reach.
Explore VHH DiscoveryResources
1