Bethyl Laboratories
Rabbit anti-DDX10 Antibody Affinity Purified
Primary
Catalog #A300-617A
TargetDDX10
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
DDX10 is a member of the DEAD box family of proteins that possesses several conserved motifs which include the highly conserved DEAD (Asp-Glu-Ala-Asp) amino acid sequence motif. The major activity of DEAD box proteins is to function as ATP-dependent RNA helicases. As helicases, DEAD proteins play an important role in all aspects of RNA metabolism and function which include pre-mRNA splicing, RNA synthesis, RNA degradation, RNA export, RNA translation, RNA secondary structure formation, ribosome biogenesis, and the assembly of RNP complexes. DDX10 may play a particular role in ribosome assembly. DDX10 has been observed to be involved in a chromosomal translocation and fused with the nucleoporin gene, NUP98, in myeloid malignancies.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 825 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetDDX10
Antigen Species
Human
Gene ID1662
Gene Symbol
DDX10
Gene Name
DEAD-box helicase 10
Uniprot IDQ13206
Protein Name
Probable ATP-dependent RNA helicase DDX10
Product Information
Applications
IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to DDX10 immobilized on solid support.
The epitope recognized by A300-617A maps to a region between residue 825 and the C-terminus (residue 875) of human DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 10 using the numbering given in entry NP_004389.2 (GeneID 1662).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A300-617A · Lot 1 (current lot)Open
Product DatasheetPDF · A300-617A · Lot 1 (current lot)Open
Safety Data SheetPDF · A300-617AOpen
Citations / Publications
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