Bethyl Laboratories
Rabbit anti-DDX18 Antibody Affinity Purified
Primary
Catalog #A300-636A
TargetDDX18
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
DDX18 is a member of the DEAD box family of proteins that possesses several conserved motifs which include the highly conserved DEAD (Asp-Glu-Ala-Asp) amino acid sequence motif. The major activity of DEAD box proteins is to function as ATP-dependent RNA helicases. As helicases, DEAD proteins play an important role in all aspects of RNA metabolism and function which include pre-mRNA splicing, RNA synthesis, RNA degradation, RNA export, RNA translation, RNA secondary structure formation, ribosome biogenesis, and the assembly of RNP complexes. DDX18 has been observed to be a target of myc-max heterodimers.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 620 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetDDX18
Antigen Species
Human
Gene ID8886
Gene Symbol
DDX18
Gene Name
DEAD-box helicase 18
Uniprot IDQ9NVP1
Protein Name
ATP-dependent RNA helicase DDX18
Product Information
Applications
IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to DDX18 immobilized on solid support.
The epitope recognized by A300-636A maps to a region between residue 625 and the C-terminus (residue 670) of human DEAD (Asp-Glu-Ala-Asp) Box Protein 18 using the numbering given in entry NP_006764.3 (GeneID 8886).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A300-636A · Lot 1 (current lot)Open
Product DatasheetPDF · A300-636A · Lot 1 (current lot)Open
Safety Data SheetPDF · A300-636AOpen
Citations / Publications
Services
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