Bethyl Laboratories
Rabbit anti-SRA Antibody Affinity Purified
Primary
Catalog #A300-743A
TargetSRA
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
Steroid receptor RNA activator 1 (SRA) was originally identified as a functional non-coding RNA that has been shown to be a coactivator of steroid receptors for androgens (AR), estrogens (ER), glucocorticoids, and progestins (PR). SRA exists in ribonucleoprotein complexes with steroid receptor coactivators (SRCs) and steroid receptors and may modulate transcriptional specificity. A 236 amino-acid protein encoded by an SRA-RNA isoform has also been identified. The SRA protein (SRAP) is widely expressed in breast cancer cell lines and breast cancer and has been demonstrated to modify the activity of the estrogen receptor.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 174 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetSRA
Antigen Species
Human
Gene ID10011
Gene Symbol
SRA1
Gene Name
steroid receptor RNA activator 1
Uniprot IDQ9HD15
Protein Name
Steroid receptor RNA activator 1
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to SRA immobilized on solid support.
The epitope recognized by A300-743A maps to a region between residue 180 and the C-terminus (residue 237) of human Steroid Receptor RNA Activator 1 using the numbering given in entry NP_001030312.1 (GeneID 10011).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A300-743A · Lot 1 (current lot)Open
Product DatasheetPDF · A300-743A · Lot 1 (current lot)Open
Safety Data SheetPDF · A300-743AOpen
Citations / Publications
Services
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