Bethyl Laboratories
Rabbit anti-RCD8 Antibody Affinity Purified
Primary
Catalog #A300-745A
TargetRCD8
HostRabbit
ClonalityPolyclonal
ApplicationIP, WB
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Product Description
RCD8 has been identified as part of an mRNA decapping complex that includes the hDcp1, and hDcp2 decapping enzyme subunits. RCD8 is important to the functional interaction between hDcp1 and hDcp2 that is essential for regulated mRNA degradation and turnover. RCD8 has also been identified as an autoantigen in human serum.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1 and 50
Isotype
IgG
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetRCD8
Antigen Species
Human
Gene ID23644
Gene Symbol
EDC4
Gene Name
enhancer of mRNA decapping 4
Uniprot IDQ6P2E9
Protein Name
Enhancer of mRNA-decapping protein 4
Alternate Names
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to RCD8 immobilized on solid support.
The epitope recognized by A300-745A maps to a region between residue 1 and 50 of human RCD8 Autoantigen using the numbering given in entry AAH43616 (GeneID 23644).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:1000 - 1:5000
Documents
Product DatasheetPDF · A300-745A · Lot 4 (current lot)Open
Product DatasheetPDF · A300-745A · Lot 4 (current lot)Open
Safety Data SheetPDF · A300-745AOpen
Citations / Publications
Services
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