Bethyl Laboratories
Rabbit anti-NCOA62 Antibody Affinity Purified
Primary
Catalog #A300-784A
TargetNCOA62
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC, IP, WB
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Product Description
NCOA62 is an integral component of the splicesome that functions in pre-mRNA splicing. NCOA62 has also been demonstrated to function as a coactivator of the Notch transcription factor and the vitamin D nuclear receptor. Additionally, NCOA2 exhibits transcriptional repression activity and associates with the N-Cor and SMRT corepressors. Recent evidence suggests a role for NCOA62 in transcription elongation.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 486 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetNCOA62
Antigen Species
Human
Gene ID22938
Gene Symbol
SNW1
Gene Name
SNW domain containing 1
Uniprot IDQ13573
Protein Name
SNW domain-containing protein 1
Product Information
Applications
IHC, IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to NCOA62 immobilized on solid support.
The epitope recognized by A300-784A maps to a region between residue 450 and 500 of human Nuclear Receptor Coactivator, 62 kD (SKIIP, SKI Interacting Protein) using the numbering given in entry NP_036377.1 (GeneID 22938).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:500 - 1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A300-784A · Lot 1 (current lot)Open
Product DatasheetPDF · A300-784A · Lot 1 (current lot)Open
Safety Data SheetPDF · A300-784AOpen
Citations / Publications
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