Bethyl Laboratories
Rabbit anti-DHX33 Antibody Affinity Purified
Primary
Catalog #A300-800A
TargetDHX33
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
DHX33 is a member of the DEAH box family of proteins that possesses several conserved motifs which include the highly conserved DEAH (Asp-Glu-Ala-His) amino acid sequence motif. The major activity of DEAH box proteins is to function as ATP-dependent RNA helicases. RNA helicases play an important role in all aspects of RNA metabolism and function which include pre-mRNA splicing, RNA synthesis, RNA degradation, RNA export, RNA translation, RNA secondary structure formation, ribosome biogenesis, and the assembly of RNP complexes. The specific function of DHX33 has not been determined.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 657 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetDHX33
Antigen Species
Human
Gene ID56919
Gene Symbol
DHX33
Gene Name
DEAH-box helicase 33
Uniprot IDQ9H6R0
Protein Name
Putative ATP-dependent RNA helicase DHX33
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to DHX33 immobilized on solid support.
The epitope recognized by A300-800A maps to a region between residue 650 and the C-terminus (residue 707) of human DEAH (Asp-Glu-Ala-His) Box Polypeptide 33 using the numbering given in entry NP_064547.2 (GeneID 56919).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A300-800A · Lot 2 (current lot)Open
Product DatasheetPDF · A300-800A · Lot 2 (current lot)Open
Safety Data SheetPDF · A300-800AOpen
Citations / Publications
Services
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