Bethyl Laboratories
Rabbit anti-SUPT6H Antibody Affinity Purified
Primary
Catalog #A300-801A
TargetSUPT6H
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC, IP, WB
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Product Description
Transcription elongation is one step in the multistage process of transcription that involves the polymerization of nucleoside monophosphates into a ribonucleotide transcript catalyzed by the multisubunit complex RNA Pol II. Transcriptional regulation involves positive factors that promote sufficient elongation rates and processivity as well as negative elongation factors that repress transcription elongation. SUPT6H is hypothesized to play a role in enhancing the rate of transcription elongation in association with RNA Pol II by mobilizing histones and modulating chromatin structure.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 425 and 475
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetSUPT6H
Antigen Species
Human
Gene ID6830
Gene Symbol
SUPT6H
Gene Name
SPT6 homolog, histone chaperone
Uniprot IDQ7KZ85
Protein Name
Transcription elongation factor SPT6
Product Information
Applications
IHC, IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to SUPT6H immobilized on solid support.
The epitope recognized by A300-801A maps to a region between residue 425 and 475 of human Suppressor of Ty 6 Homolog using the numbering given in entry NP_003161.2 (GeneID 6830).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:100 - 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A300-801A · Lot 2 (current lot)Open
Product DatasheetPDF · A300-801A · Lot 2 (current lot)Open
Safety Data SheetPDF · A300-801AOpen
Citations / Publications
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