Bethyl Laboratories
Rabbit anti-RCC2 Antibody Affinity Purified
Primary
Catalog #A300-873A
TargetRCC2
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC, WB
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Product Description
RCC2 (regulator of chromosome condensation 2) is a member of the RCC1 family of guanine nucleotide exchange factors. RCC2 has been identified as part of the chromosomal passenger complex (CPC) which coordinates the chromosomal and cytoskeletal events of mitosis. Chromosomal passengers are proteins present in complexes with aurora B, inner centromeric protein (INCENP), survivin, and borealin. RCC2 has been demonstrated to be required for the recruitment of survivin and aurora B and is essential for proper chromosome segregation and the completion of cell cleavage. Loss of RCC2 inhibits spindle assembly, activates the spindle assembly checkpoint, and blocks cells in prometaphase.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 472 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetRCC2
Antigen Species
Human
Gene ID55920
Gene Symbol
RCC2
Gene Name
regulator of chromosome condensation 2
Uniprot IDQ9P258
Protein Name
Protein RCC2
Product Information
Applications
IHC, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to RCC2 immobilized on solid support.
The epitope recognized by A300-873A maps to a region between residue 475 and the C-terminus (residue 522) of human Regulator of Chromosome Condensation 2 using the numbering given in entry NP_061185.1 (GeneID 55920).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Immunohistochemistry (IHC)1:200 - 1:1000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A300-873A · Lot 1 (current lot)Open
Product DatasheetPDF · A300-873A · Lot 1 (current lot)Open
Safety Data SheetPDF · A300-873AOpen
Citations / Publications
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