Bethyl Laboratories
Rabbit anti-CTNND1 Antibody Affinity Purified
Primary
Catalog #A300-978A
TargetCTNND1
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC, IP, WB
Image 1 of 2
local_shipping
Order within 16 minutes for shipment by today
Product Description
CTNND1 is an Armadillo repeat domain protein and member of the p120 protein family. The major function of CTNND1 involves the regulation of cadherins to influence cell-cell interactions. CTNND1 regulates cadherins by controlling cadherin turnover and/or trafficking. CTNND1 has also been found to play a role in the regulation of the actin cytoskeleton by modulating the activity of small GTPase Rho family members. CTNND1 is a substrate of the nonreceptor tyrosine kinase src and may play a role in the regulation of factors that regulate transcription.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 918 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetCTNND1
Antigen Species
Human
Gene ID1500
Gene Symbol
CTNND1
Gene Name
catenin delta 1
Uniprot IDO60716
Protein Name
Catenin delta-1
Alternate Names
Product Information
Applications
IHC, IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to CTNND1 immobilized on solid support.
The epitope recognized by A300-978A maps to a region between residue 891 and the C-terminus (residue 941) of human catenin (cadherin-associated protein), delta 1 using the numbering given in entry NP_001322.1 (GeneID 1500).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:200 - 1:1000. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A300-978A · Lot 1 (current lot)Open
Product DatasheetPDF · A300-978A · Lot 1 (current lot)Open
Safety Data SheetPDF · A300-978AOpen
Services
Antibody Conjugation
Antibody and conjugate formats developed together, built to your specification.
Request CustomizationCustom Monoclonal Antibody Discovery Services
Full custom monoclonal development, from immunization through validation.
Explore Monoclonal DevelopmentVHH Discovery
Single-domain antibodies for structurally difficult targets a conventional monoclonal can't reach.
Explore VHH DiscoveryResources
1