Bethyl Laboratories
Rabbit anti-AKAP8/AKAP95 Antibody Affinity Purified
Primary
Catalog #A301-062A
TargetAKAP8/AKAP95
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC, IP, WB
Image 1 of 2
local_shipping
Order within 4 hours and 27 minutes for shipment by today
Product Description
AKAP8/AKAP95 has been characterized as a 95 kDa protein kinase A (PKA)-anchoring protein and regulatory subunit of PKA that functions to recruit the condensin complex for chromosome condensation during mitosis. A role in DNA replication has also been demonstrated through its interaction with the pre-replication complex component, MCM2 (mini-chromosome maintenance 2). Inhibition of AKAP8/AKAP95 association with MCM2 inhibits replication initiation and elongation in vitro. AKAP8/AKAP95 has also been shown to be involved in apoptosis. KAP8/AKAP95 associates with caspase 3 and serves as a carrier to transport caspase 3 from the cytoplasm to the nucleus to induce morphological changes in the nucleus.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 642 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetAKAP8/AKAP95
Antigen Species
Human
Gene ID10270
Gene Symbol
AKAP8
Gene Name
A-kinase anchoring protein 8
Uniprot IDO43823
Protein Name
A-kinase anchor protein 8
Alternate Names
Product Information
Applications
IHC, IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to AKAP8/AKAP95 immobilized on solid support.
The epitope recognized by A301-062A maps to a region between residue 642 and the C-terminus (residue 692) of human A-kinase anchor protein 8 using the numbering given in entry NP_005849.1 (GeneID 10270).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:200 - 1:1000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A301-062A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-062A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-062AOpen
Citations / Publications
Services
Antibody Conjugation
Antibody and conjugate formats developed together, built to your specification.
Request CustomizationCustom Monoclonal Antibody Discovery Services
Full custom monoclonal development, from immunization through validation.
Explore Monoclonal DevelopmentVHH Discovery
Single-domain antibodies for structurally difficult targets a conventional monoclonal can't reach.
Explore VHH DiscoveryResources
1